Analisis Rendemen dan Skrining Senyawa Metabolit Sekunder Ekstrak Metanol Daun Violces (Viola odorata)
DOI:
https://doi.org/10.55123/insologi.v2i3.2096Keywords:
Violces (Viola Odorata), Metanol, Maceration, Yield, Phytochemical ScreeningAbstract
Violces (Viola odorata) is an herbal plant from the Violaceae family and is used in traditional medicine. This is believed to be due to the presence of phytochemical compounds in the Violces plant that provide the pharmacological activity. This study aims to determine the extract yield value and chemical compound groups contained in Violces leaves. The research method consists of four stages, namely extraction, evaporation, yield value calculation, and phytochemical screening. Violces leaf extract was obtained by maceration for 3 days using methanol as the solvent, then filtered and concentrated using a rotary evaporator. The concentrated extract of Violces leaves was then used to calculate the yield value and subjected to phytochemical screening for alkaloids, flavonoids, tannins, saponins, steroids and terpenoids. The method used for the identification of secondary metabolite compounds involve the use of chemical reagents, indicated by color changes, precipitate formation, foam/bubbles, and rings. The results of the study showed a methanol extract yield of Violces leaves of 26,70%. The phytochemical screening test of the methanol extract of Violces leaves revealed the presence of tannins, saponins and steroids.
Downloads
References
Amabye, T. G., Bezabh, A. M., & Mekonen, F. (2016). Phytochemical and Antimicrobial Potentials Leaves Extract of Eucalyptus globulus Oil from Maichew Tigray Ethiopia. International Journal of Complementary & Alternative Medicine, 2(3), 1-4. Doi:10.15406/ijcam.2016.02.00056.
Badriyah, L., & Farihah, D. A. (2022). Analisis ekstraksi kulit bawang merah (Allium cepa L.) menggunakan metode maserasi. Jurnal Sintesis: Penelitian Sains Terapan Dan Analisisnya, 3(1), 30-37.
Ebrahimzadeh, M. A., Nabavi, S. M., Nabavi, S. N., Bahramian, F., & Bekhradnia, A. R. (2010). Antioxidant And Free Radical Scavenging Activity Of H. officinalis L. var. angustifolia, V. odorata, B. hyrcana, And C. speciosum,. Pak. J. Pharm. Sci., 23(1), 29-34.
Faizal, A., & Geelen, D. (2013). Saponins and their role in biological processes in plants. Phytochemistry Reviews, 12(4), 877–893. Doi:10.1007/s11101-013-9322-4
Ghasemzadeh, A., Jaafar, H. Z. E., Bukhori, M. F. M., Rahmat, M. H., & Rahmat, A. (2018). Assessment and comparison of phytochemical constituents and biological activities of bitter bean (Parkia speciosa Hassk.) collected from different locations in Malaysia. Chemistry Central Journal, 12(1). https://doi.org/10.1186/s13065-018-0377-6
Handoyo, D. L. Y. (2020). Pengaruh Lama Waktu Maserasi (Perendaman) Terhadap Kekentalan Ekstrak Daun Sirih (Piper betle), Jurnal Farmasi Tinctura, 2(1), 34-41.
Haralkar, K. V, & Biradar, S. R. (2020). Preliminary Phytochemical screening of leaf and root of Viola odorata L. OUR HERITAGE, 68(5), 428-432.
Hoang, T. N. N, Phan, T. T., Phan, T. K. L., Nguyen, N. H. V., Dong, T. A. D., & Le, T. H. A. (2023). Phytochemical Screening, Extraction, and Determination of the Bioactivities of the Extract-Enriched Polyphenols and Saponins from Musa balbisiana Fruit. Journal of Food Processing and Preservation, Volume 2023, 1–16. Doi:10.1155/2023/2581641.
Hussein, R. A., & A. El-Anssary, A. (2019). Plants Secondary Metabolites: The Key Drivers of the Pharmacological Actions of Medicinal Plants. Herbal Medicine IntechOpen, 11-30. Doi:10.5772/intechopen.76139.
Iqbal, E., Salim, K. A., & Lim, L. B. L. (2015). Phytochemical screening, total phenolics and antioxidant activities of bark and leaf extracts of Goniothalamus velutinus (Airy Shaw) from Brunei Darussalam. Journal of King Saud University - Science, 27(3), 224–232. Doi:10.1016/j.jksus.2015.02.003.
Kabera, J. N., Semana, E., Mussa, A. R., & He, X. (2014). Plant Secondary Metabolites: Biosynthesis, Classification, Function and Pharmacological Properties. Journal of Pharmacy and Pharmacology, Vol. 2, 377-392.
Kurniawati, A. (2019). Pengaruh Jenis Pelarut Pada Proses Ekstraksi Bunga Mawar Dengan Metode Maserasi Sebagai Aroma Parfum. Journal of Creativity Student, 2(2), 74-83.
Mittal, P., Gupta, V., Goswami, M., Thakur, N., & Bansal, P. (2015). Phytochemical And Pharmacological Potential Of Viola Odorata, IJP:International Journal of Pharmacognosy, 2(5), 215-220. Doi:10.13040/IJPSR.0975-8232.IJP.
Mulla, İ., Roqaiya, M., & Imran Khan, M. (2019). Efficacy of Viola odorata flower decoction in chronic rhinosinusitis. Medical Journal of Islamic World Academy of Sciences, 27(3), 77–84. Doi:10.5505/ias.2019.08379.
Nahor, E. M., Rumagit, B. I., Tou, H. Y. (2020). Perbandingan Rendemen Ekstrak Etanol Daun Andong (Cordyline futicosa L.) Menggunakan Metode Ekstraksi Maserasi dan Sokhletasi. Prosiding Seminar Nasional 2020, Manado 2 Desember 2020, 40-44.
Oktavia, F. D., & Sutoyo, S. (2021). Skrining Fitokimia, Kandungan Flavonoid Total, Dan Aktivitas Antioksidan Ekstrak Etanol Tumbuhan Selaginella doederleinii. Jurnal Kimia Riset, 6(2), 141-153.
Patil, S. R., Patil, R. S., & Godghate, A. G. (2016). Mentha piperita Linn: Phytochemical, antibacterial and dipterian adulticidal approach. International Journal of Pharmacy and Pharmaceutical Sciences, 8(3), 352-355.
Rasyid, Z. A., Farida, A., Daud, S. H., Wiwin, S., Wijaya, K. I., & Tangke, A. E. (2020). Bioactivities of forest medicinal plants on kutai ethnic (Indonesia) of tapak leman (Hippobroma longiflora (L) G. Don). GSC Biological and Pharmaceutical Sciences, 11(2), 91–98. Doi:10.30574/gscbps.2020.11.2.0125.
Senduk, T. W., Montolalu, L. A. D. Y., & Dotulong, V. (2020). Rendemen Ekstrak Air Rebusan Daun Tua Mangrove Sonneratia alba. Jurnal Perikanan dan Kelautan Tropis, 11(1), 9-15.
Setyorini, D., & Antarlina, S. S. (2022). Secondary metabolites in sorghum and its characteristics. In Food Science and Technology, Volume 42, 1-13. Doi:10.1590/fst.49822.
Shaikh, J. R., & Patil, M. (2020). Qualitative tests for preliminary phytochemical screening: An overview. International Journal of Chemical Studies, 8(2), 603–608. Doi:10.22271/chemi.2020.v8.i2i.8834.
Sharma, T., Pandey, B., Shrestha, B. K., Koju, G. M., Thusa, R., & Karki, N. (2020). Phytochemical Screening of Medicinal Plants and Study of the Effect of Phytoconstituents in Seed Germination. Tribhuvan University Journal, 35(2), 1–11. Doi:10.3126/tuj.v35i2.36183.
Singh, A., Dhariwal, S., & Navneet. (2018). Traditional uses, Antimicrobial potential, Pharmacological properties and Phytochemistry of Viola odorata: A Mini Review. The Journal of Phytopharmacology, 7(1), 103-105.
Solihah, M. A., Rosli, W., & Nurhanan, A. R. (2012). Phytochemicals screening and total phenolic content of Malaysian Zea mays hair extracts. International Food Research Journal, 19(4), 1533-1538.
Sultan, A., & Raza, A. R. (2015). Steroids: A Diverse Class of Secondary Metabolites. Medicinal Chemistry, 5(7), 310-317. Doi:10.4172/2161-0444.1000279.
Susanti, N. M. P., Budiman, I. N. A., & Warditiani, N. K. (2014). Skrining Fitokimia Ekstrak Etanol 90% Daun Katuk (Sauropus androgynus (L.). Jurnal Farmasi Udayana, 3(1), 83-86.
Tong, Z., He, W., Fan, X., & Guo, A. (2022). Biological Function of Plant Tannin and Its Application in Animal Health. Frontiers in Veterinary Science, Volume 8, 1-7. Doi:10.3389/fvets.2021.803657.
Wijaya, H., Novitasari, Jubaidah, S. (2018). Perbandingan Metode Ekstraksi Terhadap Rendemen Ekstrak Daun Rambai Laut (Sonneratia caseolaris L. Engl). Jurnal Ilmiah Manuntung, 4(1), 79-83.
Zannah, F., Amin, M., Suwono, H., & Lukiati, B. (2017). Phytochemical screening of Diplazium esculentum as medicinal plant from Central Kalimantan, Indonesia. AIP Conference Proceedings, 1844. Doi:10.1063/1.4983439.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Sandi Al Afif Fathoroni Putra, Surahmaida Surahmaida

This work is licensed under a Creative Commons Attribution 4.0 International License.
Hak cipta pada setiap artikel adalah milik penulis.
Penulis mengakui bahwa INSOLOGI (Jurnal Sains dan Teknologi) sebagai publisher yang mempublikasikan pertama kali dengan lisensi Creative Commons Attribution 4.0 International License.
Penulis dapat memasukan tulisan secara terpisah, mengatur distribusi non-ekskulif dari naskah yang telah terbit di jurnal ini kedalam versi yang lain, seperti: dikirim ke respository institusi penulis, publikasi kedalam buku, dan lain-lain. Dengan mengakui bahwa naskah telah terbit pertama kali pada INSOLOGI (Jurnal Sains dan Teknologi).
























