Chemical constituents of ripe Momordica charantia by Gas Chromatography Mass Spectrometry (GCMS)

Authors

  • Diah Miftahul Aini STIKES Kusuma Bangsa
  • Baiq Desy Ratnasari STIKES Kusuma Bangsa
  • Zul Hendry STIKES Yarsi Mataram

DOI:

https://doi.org/10.55123/insologi.v1i3.359

Keywords:

Momordica charantia, Lombok, GCMS, chemical constituents

Abstract

Momordica charantia, commonly called bitter melon, is a plant belonging to Cucurbitaceae family known for centuries for its nutritional properties. This plant have been used as folklore medicine for the management of hypertensions, cold, cough, and diabetes. The aim of the present study was to characterize the chemical constituents of ripe M. charantia from Lombok, Indonesia by Gas Chromatography Mass Spectrometry (GCMS). The method that used is maseration with ethanol as solvent. The ethanol extract of ripe M. charantia was characterized by gas chromatography-flame ionization detection and gas chromatography coupled with mass spectrometry analyses. The chemical constituent of ripe M. charantia that followed by GCMS were 2,4-Pentanediol (23,61 %), Eucalyptol (1,8-Cineole) (20,41 %), Trans (Beta)-Caryopyllene (10,07 %), 3-Decyn-2-ol  (9.54 %), Epiglobulol (7.82 %), Methyleugenol (7.15%), Beta-Selinene (5.80 %), Alpha-Selinene (5.43 %), and Alpha-Humulene (5.29 %). This is the first report on chemical screening of ethanol extract of ripe M. charantia and GC-MS analysis shows the presence of compounds which have medicinal properties.

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References

Singh AK, Behera TK, Chandel D, Sharma P, Singh NK. (2007) Assessing genetic relationships among bitter gourd (momordica charantia L.) accessions using inter-simple sequence repeat (ISSR) markers. J Hortic Sci Biotechnol. 82(2), 217-222.

Alves MS, Ribeiro GM, Souza AN, Carvalho CM. (2017). First report of a 16SrIII-B phytoplasma associated with Momordica charantia witches’-broom in Brazil. Plant Dis. 101(7), 1314.

Chen F, Huang G, Huang H. (2021). Preparation, analysis, antioxidant activities in vivo of phosphorylated polysaccharide from Momordica charantia. Carbohydr Polym. 252.

Upadhyay A, Agrahari P, Singh DK. (2015). A review on salient pharmacological features of momordica charantia. International Journal of Pharmacology. 11(5), 405-413.

Desai S, Tatke P, Mane T, Gabhe S. (2021). Isolation, characterization and quantitative HPLC-DAD analysis of components of charantin from fruits of Momordica charantia. Food Chem. 345.

Khan A, Raghunathan V, Singaravelu DL, Sanjay MR, Siengchin S, Jawaid M, et al. (2020). Extraction and Characterization of Cellulose Fibers from the Stem of Momordica Charantia. J Nat Fibers.https://doi.org/10.1080/15440478.2020.1807442

Jiang B, Ji M, Liu W, Chen L, Cai Z, Zhao Y, et al. (2016). Antidiabetic activities of a cucurbitane-type triterpenoid compound from Momordica charantia in alloxan-induced diabetic mice. Mol Med Rep.14(5), 4865-4872.

Dwijayanti DR, Shimada T, Ishii T, Okuyama T, Ikeya Y, Mukai E, et al. (2020). Bitter melon fruit extract has a hypoglycemic effect and reduces hepatic lipid accumulation in ob/ob mice. Phyther Res. 34(6), 1338-1346.

Juergens LJ, Worth H, Juergens UR. (2020). New Perspectives for Mucolytic, Anti-inflammatory and Adjunctive Therapy with 1,8-Cineole in COPD and Asthma: Review on the New Therapeutic Approach. Advances in Therapy. 37(5), 1737-1753.

Lee HS, Park DE, Song WJ, Park HW, Kang HR, Cho SH, et al. (2016). Effect of 1.8-Cineole in Dermatophagoides pteronyssinus-stimulated bronchial epithelial cells and mouse model of asthma. Biol Pharm Bull. 39(6),946-952.

Chograni H, Riahi L, Dhahri S, Ezzine O, Chakroun H, Messaoud C. (2021). Interspecific variability of 1,8-cineole content, phenolics and bioactivity among nine Eucalyptus taxa growing under the sub-humid bioclimate stage. J Complement Integr Med. 17(4).

Cai ZM, Peng JQ, Chen Y, Tao L, Zhang YY, Fu LY, et al. (2020).1,8-Cineole: a review of source, biological activities, and application. Journal of Asian Natural Products Research. 23(10), 938-954.

Goenharto S, Sudiana IK, Salim S, Narmada IB. (2020). Protective activity of 1, 8-cineole against inflammatory and oxidative stress marker from methyl methacrylate exposure. J Glob Pharma Technol. 12(1), 135-141.

Tremmel R, Herrmann K, Engst W, Meinl W, Klein K, Glatt H, et al. (2017). Methyleugenol DNA adducts in human liver are associated with SULT1A1 copy number variations and expression levels. Arch Toxicol. 91(10), 3329-3339.

Yang X, Feng Y, Zhang Z, Wang H, Li W, Wang DO, et al. (2020). In Vitro and in Vivo Evidence for RNA Adduction Resulting from Metabolic Activation of Methyleugenol. J Agric Food Chem. 68(51), 15134-15141.

Alminderej F, Bakari S, Almundarij TI, Snoussi M, Aouadi K, Kadri A. (2020). Antioxidant activities of a new chemotype of piper cubeba L. Fruit essential oil (methyleugenol/eugenol): In silico molecular docking and admet studies. Plants. 9(11), 1-18.

Karakaya S, Göger G, Kiliç CS, Demirci B. (2016). Composition of volatile oil of the aerial parts, flowers and roots of Ferulago blancheana post. (Apiaceae) growing in Turkey and determination of their antimicrobial activities by bioautography method. Turkish J Pharm Sci.13(2), 173-180.

Rashid MMO, Akhter KN, Chowdhury JA, Hossen F, Hussain MS, Hossain MT. (2017). Characterization of phytoconstituents and evaluation of antimicrobial activity of silver-extract nanoparticles synthesized from Momordica charantia fruit extract. BMC Complement Altern Med. 17(1).

Cortez-Navarrete M, Martínez-Abundis E, Pérez-Rubio KG, González-Ortiz M, Méndez-Del Villar M. (2018). Momordica charantia administration improves insulin secretion in type 2 diabetes mellitus. J Med Food. 21(7), 672-677.

Mozaniel S de O, Wanessa A da C, Fernanda WFB, Marilena EA, Gracialda CF, Raul N de CJ. (2018). Phytochemical profile and biological activities of Momordica charantia L. (Cucurbitaceae): A review. African J Biotechnol. 17(27), 829-846.

Joseph B, Jini D. (2018). Antidiabetic effects of Momordica charantia (bitter melon) and its medicinal potency. Asian Pacific J Trop Dis. 3(2), 93-102.

Offor U, Naidu EC, Ogedengbe OO, Jegede AI, Peter AI, Azu OO. (2018). Nephrotoxicity and highly active antiretroviral therapy: Mitigating action of Momordica charantia. Toxicol Reports. 5, 1153-1160.

Ng ZX, Kuppusamy UR. (2019). Effects of different heat treatments on the antioxidant activity and ascorbic acid content of bitter melon, Momordica charantia. Brazilian J Food Technol. 22. DOI 10.1590/1981-6723.28318

Osonuga Odusoga A, Osonuga Ifabunmi O, Ayokunle O. (2014). Evaluation of oral administration of aqueous leaf extract of Momordica charantia on fertility hormones of adult male wistar rats. Glob J Pharmacol. 8(2), 150-153.

Tumkiratiwong P, Ploypattarapinyo R, Pongchairerk U, Thong-Asa W. (2014). Reproductive toxicity of Momordica charantia ethanol seed extracts in male rats. Iran J Reprod Med. 12(10), 695- 704.

J. Martins, C. Costa, T. Oliveira, R. Gonçalves, and F. Branco, “How smartphone advertising influences consumers’ purchase intention,” Journal of Business Research, vol. 94, no. August 2017, pp. 378–387, 2019.

Canalys, “Global smartphone market share Q2 2021,” Canalys estimates (sell-in shipments), Smartphone Analysis, July 2021, 2021. [Online]. Available: https://www.canalys.com/newsroom/worldwide-smartphone-market-q2-2021. [Accessed: 10-Aug-2021].

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Published

2022-06-28

How to Cite

Aini, D. M., Ratnasari, B. D., & Hendry, Z. (2022). Chemical constituents of ripe Momordica charantia by Gas Chromatography Mass Spectrometry (GCMS). INSOLOGI: Jurnal Sains Dan Teknologi, 1(3), 189–194. https://doi.org/10.55123/insologi.v1i3.359

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