Aktivitas Antibakteri Ekstrak Daun Bidara (Ziziphus mauritiana) terhadap Pertumbuhan Staphylococcus aureus pada Isolat Ulkus Diabetikum
DOI:
https://doi.org/10.55123/insologi.v5i3.8145Keywords:
Diabetes Mellitus, Bidara, Staphylococcus AureusAbstract
Diabetes mellitus is a long term metabolic disease that may trigger various complications, including diabetic ulcers. These ulcers are highly vulnerable to bacterial infection, especially by Staphylococcus aureus, which can delay wound healing and worsen the patient’s condition. This study was conducted to determine the antibacterial activity of ethanol extract of bidara leaves (Ziziphus mauritiana L.) against Staphylococcus aureus isolated from diabetic ulcer wounds. The research employed an experimental laboratory design using the well diffusion technique on Mueller Hinton Agar media. Bidara leaf extract was tested at concentrations of 5%, 10%, and 15%. Chloramphenicol was applied as the positive control, while DMSO and distilled water were used as negative and neutral controls. Antibacterial effectiveness was assessed based on the diameter of the inhibition zone formed around each well. The findings demonstrated that the ethanol extract of bidara leaves was able to inhibit the growth of Staphylococcus aureus at all tested concentrations. The strongest inhibitory effect was observed at the 15% concentration. Statistical evaluation using one way ANOVA revealed a significant difference between treatment groups (p<0.05). The antibacterial activity is thought to be associated with the presence of bioactive compounds, including flavonoids, alkaloids, tannins, and saponins, which may interfere with bacterial cell function and growth. In summary, ethanol extract of bidara leaves shows promising potential as a natural antibacterial agent against Staphylococcus aureus isolated from diabetic ulcers.
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Anibogwu, R., Jesus, K. De, Pradhan, S., Pashikanti, S., Mateen, S., & Sharma, K. (2021). Extraction, Isolation and Characterization of Bioactive Compounds from Artemisia and Their Biological Significance: A Review. Molecules (Basel, Switzerland), 26(22). https://doi.org/10.3390/molecules26226995
Choudhury, A. (2022). Potential Role of Bioactive Phytochemicals in Combination Therapies against Antimicrobial Activity. Journal of Pharmacopuncture, 25(2), 79–87. https://doi.org/10.3831/KPI.2022.25.2.79
Dias, M. C., Pinto, D. C. G. A., & Silva, A. M. S. (2021). Plant Flavonoids: Chemical Characteristics and Biological Activity. Molecules, 26(17). https://doi.org/10.3390/molecules26175377
Du, F., Ma, J., Gong, H., Bista, R., Zha, P., Ren, Y., Gao, Y., Chen, D., Ran, X., & Wang, C. (2022). Microbial Infection and Antibiotic Susceptibility of Diabetic Foot Ulcer in China: Literature Review. Frontiers in Endocrinology, Volume 13. https://doi.org/10.3389/fendo.2022.881659
Ghanimi, R., Ouhammou, A., Ahouach, A., & Cherkaoui, M. (2022). Ethnobotanical study on wild edible plants traditionally used by Messiwa people, Morocco. Journal of Ethnobiology and Ethnomedicine, 18(1), 16. https://doi.org/10.1186/s13002-022-00500-4
Hossain, T. J. (2024). Methods for screening and evaluation of antimicrobial activity: A review of protocols, advantages, and limitations. European Journal of Microbiology & Immunology, 14(2), 97–115. https://doi.org/10.1556/1886.2024.00035
Li, J., & Monje-Galvan, V. (2023). In Vitro and In Silico Studies of Antimicrobial Saponins: A Review. In Processes (Vol. 11, Number 10, p. 2856). https://doi.org/10.3390/pr11102856
Macdonald, K. E., Boeckh, S., Stacey, H. J., & Jones, J. D. (2021). The microbiology of diabetic foot infections: a meta-analysis. BMC Infectious Diseases, 21(1), 770. https://doi.org/10.1186/s12879-021-06516-7
Mancuso, G., Midiri, A., Gerace, E., & Biondo, C. (2021). Bacterial Antibiotic Resistance: The Most Critical Pathogens. Pathogens (Basel, Switzerland), 10(10). https://doi.org/10.3390/pathogens10101310
Mardhiyani, D., & Afriani, M. (2021). Antibacterial Activity Test Of Leaves Bidara (Ziziphus mauritiana Lam) Ethanolic Extracts Against Staphylococcus aureus. JURNAL PROTEKSI KESEHATAN, 10(1), 44–48. https://doi.org/10.36929/jpk.v10i1.343
Mohamadou, S., James, B., Roger, D. D., Francky Steve, N. S., & Leopold, T. N. (2021). Phytochemical Screening and Antimicrobial Activity of Ziziphus mauritiana Lam. And Ziziphus mucronate Lam. Extracts. Journal of Advances in Medical and Pharmaceutical Sciences, 23(8 SE-Original Research Article), 25–37. https://doi.org/10.9734/jamps/2021/v23i830252
Muscolo, A., Mariateresa, O., Giulio, T., & Mariateresa, R. (2024). Oxidative Stress: The Role of Antioxidant Phytochemicals in the Prevention and Treatment of Diseases. International Journal of Molecular Sciences, 25(6). https://doi.org/10.3390/ijms25063264
Pauloi, L., & Salim, R. M. (2025). Exploring the antimicrobial potential from various parts of Ziziphus mauritiana Lam. Scientific Reports, 15(1), 1–14. https://doi.org/10.1038/s41598-025-20756-6
Rafiq, A. R., & Ameen, A. (2025). Antimicrobial activity of Ziziphus mauritiana and Acacia nilotica against multidrug-resistant Escherichia coli and Klebsiella pneumoniae. AIMS Molecular Science, 12(3), 234–254. https://doi.org/10.3934/molsci.2025015
Saraswati, A. T., Sugihartuti, R., Puspitasari, Y., Witaningrum, A. M., Rachmawati, K., Maslachah, L., Raharjo, H. M., Hisyam, M. A. M., & Tacharina, M. R. (2023). Antibacterial Activity of Bidara Leaf Extract (Ziziphus mauritiana) Against Staphylococcus aureus Isolated from Mastitis Case In Vitro. Journal of Basic Medical Veterinary, 12(2 SE-Original Research), 85–91. https://doi.org/10.20473/jbmv.v12i2.51106
Syahriel, D., Hervina, H., & Sardi, N. W. A. (2025). Antibacterial Activity of Bidara Arab Leaves (Ziziphus spina-christi L) Extract Against Gram-negative Anaerobic Subgingival Bacteria. Odonto : Dental Journal; Vol 11, No 2 (2024): December 2024 https://jurnal.unissula.ac.id/index.php/odj/article/view/38563
Vaou, N., Stavropoulou, E., Voidarou, C. (., Tsakris, Z., Rozos, G., Tsigalou, C., & Bezirtzoglou, E. (2022). Interactions between Medical Plant-Derived Bioactive Compounds: Focus on Antimicrobial Combination Effects. In Antibiotics (Vol. 11, Number 8, p. 1014). https://doi.org/10.3390/antibiotics11081014
Vitale, S., Colanero, S., Placidi, M., Di Emidio, G., Tatone, C., Amicarelli, F., & D’Alessandro, A. M. (2022). Phytochemistry and Biological Activity of Medicinal Plants in Wound Healing: An Overview of Current Research. Molecules (Basel, Switzerland), 27(11). https://doi.org/10.3390/molecules27113566
Wada, F. W., Mekonnen, M. F., Sawiso, E. D., Kolato, S., Woldegiorgis, L., Kera, G. K., El-Khatib, Z., Ashuro, A. A., Biru, M., & Boltena, M. T. (2023). Bacterial profile and antimicrobial resistance patterns of infected diabetic foot ulcers in sub-Saharan Africa: a systematic review and meta-analysis. Scientific Reports, 13(1), 14655. https://doi.org/10.1038/s41598-023-41882-z
Yuziani, Y., Agtari, Y., & Novalia, V. (2025). Uji Zona Hambat Dan Uji Beda Ekstrak Etanol Daun Bidara (Z. Mauritiana Lamm) Terhadap Bakteri Staphylococcus Aureus. JFM (Jurnal Farmasi Malahayati), 8(1), 13–22. https://doi.org/10.33024/jfm.v8i1.14427
Zhang, X.-Z., Chen, M.-X., Hou, R., Wang, W.-Q., He, Z.-D., You, J.-S., & Song, X. (2026). Natural Alkaloids as Antimicrobial Agents: Mechanisms, Potentials and Challenges. Molecules (Basel, Switzerland), 31(7). https://doi.org/10.3390/molecules31071204
Zhou, H., Chen, L., Ouyang, K., Zhang, Q., & Wang, W. (2023). Antibacterial activity and mechanism of flavonoids from Chimonanthus salicifolius S. Y. Hu. and its transcriptome analysis against Staphylococcus aureus. Frontiers in Microbiology, Volume 13. https://doi.org/10.3389/fmicb.2022.1103476
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