Aktivitas Antibakteri Kombinasi Ekstrak Etanol Kulit Kopi Robusta dan Serai Wangi terhadap Escherichia coli
DOI:
https://doi.org/10.55123/sehatmas.v5i2.7972Keywords:
Antibacterial Activity, Robusta Coffee Peel, Citronella Grass, Escherichia coli, Disc DiffusionAbstract
Antibacterial activity of a combination of 70% ethanol extracts of Robusta coffee peel (Coffea canephora) and citronella grass (Cymbopogon nardus) against Escherichia coli was evaluated using the disc diffusion (Kirby–Bauer) method. The extracts were obtained through maceration with 70% ethanol and combined in a 1:1 ratio at concentrations of 10%, 20%, 40%, 60%, and 80%. The parameter observed was the diameter of the inhibition zone formed around the discs. The results showed no antibacterial activity at concentrations of 10% and 20%. At 40%, the inhibition zone was 4.87 mm (weak category), increasing to 5.43 mm (moderate category) at 60% and 7.53 mm (moderate category) at 80%. The positive control (ciprofloxacin) exhibited an inhibition zone of 23.31 mm (very strong category), while the negative control (DMSO) showed no inhibition. Statistical analysis using One Way ANOVA indicated significant differences among treatments (p<0.05). In conclusion, the combination of 70% ethanol extracts of Robusta coffee peel and citronella grass demonstrated concentration-dependent antibacterial activity against Escherichia coli, although the activity remained in the weak to moderate category.
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Batubara, S. H., Juli, N., Simanjuntak, P., & Yunus, M. (2026). Formulasi Dan Uji Aktivitas Antibakteri Sediaan Gel Hand Sanitizer Ekstrak Etanol Daun Kumis Kucing ( Orthosiphon Stamineus ) Terhadap Bakteri. Jurnal Kesehatan Tambusai, 7(1), 1582–1595.
Bhandarkar, N. S., Mouatt, P., Majzoub, M. E., Thomas, T., Brown, L., & Panchal, and S. K. (2021). Coffee Pulp, a By-Product of Coffee Production, Modulates Gut Microbiota and Improves Metabolic Syndrome in High-Carbohydrate, High-Fat Diet-Fed Rats. Pathogens, 10. https://doi.org/ 10.3390/pathogens10111369
Dewi, N. P. K. C., Hidayati, A. R., & Hanifa, N. I. (2023). Aktivitas Antibakteri Senyawa Fenolik dari Kulit Buah Kopi Robusta (Coffea canephora L.). Unram Medical Journal, 12(3), 30–33. https://doi.org/10.29303/jk.v12i3.4443
Enggrainia, B., Leswara, D. F., & Pratama, N. P. (2024). Uji Aktivitas Antibakteri Ekstrak Etanol Sereh Wangi (Cymbopogon nardus L. Rendle) Terhadap Pertumbuhan Bakteri Staphylococcus aureus ATCC 25923 Menggunakan Metode Sumuran. Journal of Pharmaceutical (Jop), 2(1), 1–11. https://doi.org/10.30989/jop.v2i1.1442
Febrianti, Y., Samidah, I., & Tepi, D. (2022). Hubungan Antara Pengetahuan, Sikap, Dan Karakteristik Dengan Perilaku Ibu Dalam Pencegahan Diare Pada Balita Di Wilayah Kerja Puskesmas Beringin Raya Kota Bengkulu Tahun 2022. Journal of Nursing and Public Health, 10(2), 148–155.
Fitriani, L., Tuntun, M., & Marhamah, M. (2021). Uji Daya Hambat Ekstrak Serai Wangi (Cymbopogon nardus (L.) Rendl ) Terhadap Pertumbuhan Bakteri Escherichia coli ATCC 25922. Jurnal Medika Malahayati, 5(3), 191–197. https://doi.org/10.33024/jmm.v5i3.5113
Ghezelghaye, P. N., Alibegli, M., Fazelnejad, A., Davoodi, A., & Goli, H. R. (2025). Antibacterial activity of tannin-free ethanolic extracts from medicinal plants against methicillin-resistant Staphylococcus aureus. BMC Complementary Medicine and Therapies, 1, 1–8. https://doi.org/10.1186/s12906-025-05019-1%0A(2025)
Huang, J., Zaynab, M., Sharif, Y., Khan, J., Al-Yahyai, R., Sadder, M., Ali, M., Saber, Alarab, & Li, S. (2024). Tannins as antimicrobial agents: Understanding toxic effects on pathogens. Toxicon, 247. https://doi.org/10.1016/j.toxicon.2024.107812
Kementerian Kesehatan RI. (2022). Farmakope Herbal Indonesia (Edisi Ii). Kementerian Kesehatan Republik Indonesia.
Kurniawana, G., Chabibah, Rahmawati, R. P., Arif, F., & Apriliyani, F. (2023). Uji Aktifitas Antioksidan Ekstrak Etanol 70% Biji Kopi Robusta (Coffea Robusta L.) Di Kudus Dengan Metode DPPH. Indonesia Jurnal Farmasi, 8(2), 127–135.
Lee, Y., Cho, E., Maskey, S., & Nguyen, D. (2023). Value-Added Products from Coffee Waste : A Review. Molecules, 28, 1–19. https://doi.org/10.3390/ molecules28083562%0AAcademic
Lestari, I., & Hanum, G. R. (2019). Aktivitas Antibakteri Kombinasi Ekstrak Daun Mengkudu (Morinda citrifoli L.) dan Bawang Putih (Allium sativum) terhadap Escherichia coli dan Staphylococcus aureus. Medicra (Journal of Medical Laboratory Science/Technology), 2(2), 43–47. https://doi.org/10.21070/medicra.v2i2.1475
Liza, B., Haris Munandar, N., ayuk Putri, R., & Ainil Fithri, P. (2025). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Terong Hijau (Solanum Melongena) Dalam Kebocoran Dna Dan Protein Bakteri. Prepotif : Jurnal Kesehatan Masyarakat, 9(2), 5016–5025. https://doi.org/10.31004/prepotif.v9i2.45009
Makiso, M., Getachew, P., Tagesse, B., Yohannis, E., Afework, A., Meteke, N., Yasin, S., Milkias, M., Tola, Y. B., Teka, T. A., Fekadu, H., & Worku, M. (2025). Green valorization of coffee industry residues : Emerging innovations and their role in sustainable food and feed applications. Applied Food Research, 5(2), 101181. https://doi.org/10.1016/j.afres.2025.101181
Manasa, V., Padmanabhan, A., & Appaiah, K. A. A. (2020). Utilization of coffee pulp waste for rapid recovery of pectin and polyphenols for sustainable material recycle. Waste Management. https://doi.org/10.1016/j.wasman.2020.10.045
Muqaromah, A., Arfianti, M., & Pebriani, E. (2025). Hubungan Antara Pengetahuan Tentang Diare Dengan Perilaku Ibu Dalam Pencegahan Kejadian Penyakit Diare Pada Balita Diwilayah Kerja Puskesmas Jembatan Kecil Kota Bengkulu The Relationship Between Knowledge About Diarrhea And Mother ’ s Behavior In Preventi. Jurnal Kesehatan Mitra Sekawan, 1(2), 101–106.
Ningtyas, A. R., Susanti, M. E., & Aprianti, D. (2026). Hubungan Pengetahuan Personal Hygiene Ibu Dengan Kejadian Diare Pada Balita Di Wilayah Kerja Puskesmas Jembatan Kecil Kota Bengkulu Tahun 2026. Jurnal Multimedia Dehasen, 5(2), 1223–1230. https://doi.org/10.37676/mude.v5i2.10867
Odongo, E. A., Mutai, P. C., Amugune, B. K., Mungai, N. N., Akinyi, M. O., & Kimondo, J. (2023). Evaluation of the antibacterial activity of selected Kenyan medicinal plant extract combinations against clinically important bacteria. BMC Complementary Medicine and Therapies, 4, 1–9. 10.1186/s12906-023-03939-4
Priamsari, M. R., & Wibowo, A. C. (2020). Aktivitas Antibakteri Ekstrak Perasan Daun Mengkudu ( Morinda citrifolia L .) Terhadap Escherichia coli Secara In Vitro In Vitro Antibacterial Activity From Leaf Extract Feeding Of Morinda citrifolia L . AGAINST Escherichia coli. Jurnal Riset Kefarmasian Indonesia, 2(1), 26–34.
Putra, R. P., Fitri, & Patang. (2024). Pemanfataan Ekstrak Kulit Buah Kopi Robusta dalam Pengembangan Produk Minuman Cendol Instan Fungsional dengan Sifat Antioksidan Tinggi dan Kafein Rendah. Jurnal Pendidikan Teknologi Pertanian, 10, 141–154.
Ranjan, S., Asish, P., Ghanishtha, M., Swastik, P., & Rabi, B. B. (2025). Antibacterial properties and therapeutic potential of few medicinal plants : current insights and challenges. Discover Plants, 2(21). https://doi.org/10.1007/s44372-025-00097-4
Rante, H., Subehan, Wulandari, R., & Evary, Y. M. (2021). Antibacterial activity of robusta coffee (Coffea robusta L.) peel extract against human pathogenic bacteria. Journal of Experimental Biology and Agricultural Sciences, 9(Spl-2-ICOPMES_2020), S264–S268. https://doi.org/10.18006/2021.9(Spl-2-ICOPMES_2020).S264.S268
Safar, A. A., Ghafoor, A. O., & Dastan, D. (2020). Chemical Composition , Antibacterial and Antioxidant Activities of Tagetes patula L . Essential Oil Raised in Erbil , Iraq. Journal of Reports in Pharmaceutical Sciences, 9(1), 59–67. doi:10.4103/jrptps.JRPTPS_68_19
Setyobudi, R. H., Fayiz, M., Atoum, M., Damat, D., Yandri, E., Nugroho, Y. A., Susanti, M. S., Wahono, S. K., Widodo, W., Zalizar, L., Wahyudi, A., Saati, E. A., Maftuchah, M., Hussain, Z., Yono, D., Harsono, S. S., Mahaswa, R. K., Susanto, H., Adinurani, P. G., Mindarti, S. (2022). Evaluation of Coffee Pulp Waste from Coffee Cultivation Areas in Indonesia as Iron Booster. JJBS Jordan Journal of Biological Sciences, 15(3), 475–488. https://doi.org/10.54319/jjbs/150318
Summer, K., Browne, J., Hollanders, M., & Benkendorff, K. (2022). Out of control : The need for standardised solvent approaches and data reporting in antibiofilm assays incorporating dimethyl-sulfoxide ( DMSO ). Biofilm, 4(August), 100081. https://doi.org/10.1016/j.bioflm.2022.100081
Susilowati, M., Syukur, C., Riset, P., & Riset, B. (2022). Karakterisasi Beberapa Aksesi Serai Wangi ( Cymbopogon nardus L .) Asal Cianjur Characterization of Citronella ( Cymbopogon nardus L . ) Accessions from Cianjur. Jurnal Vegetalika, 11(4), 305–314.
Ulfah, M., Priyanto, W., & Prabowo, H. (2022). Kajian Kadar Air Terhadap Umur Simpan Simplisia Nabati Minuman Fungsional Wedang Rempah. Jurnal Pendidikan Dasar Dan Sosial Humaniora, 1, 1103–1112.
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