Kajian Potensi Etil Asetat Ekstrak Daun Andaliman : Total Fenol Flavonoid dan Aktivitas Uji Antioksidan
DOI:
https://doi.org/10.55123/insologi.v5i1.7537Keywords:
Zanthoxylum Acanthopodium, Ethyl Acetate Extract, Flavonoid, Phenolic Compounds, Antioxidant Activity, DPPHAbstract
Andaliman leaves (Zanthoxylum acanthopodium DC.) are a traditional plant native to North Sumatra with potential as a natural antioxidant source due to their phenolic and flavonoid compounds. This study aimed to evaluate the antioxidant potential of the ethyl acetate extract of andaliman leaves based on total phenolic content, total flavonoid content, and antioxidant activity. Extraction was carried out using the maceration method with ethyl acetate as the solvent. Total flavonoid content was determined using the AlCl₃ method with quercetin as the standard, while total phenolic content was analyzed using the Folin– Ciocalteu method with gallic acid as the standard. Antioxidant activity was evaluated using the DPPH radical scavenging assay. The results showed that the ethyl acetate extract of andaliman leaves contained 73.40 mg QE/g extract of total flavonoids (7.3%) and 148.48 mg GAE/g extract of total phenolics. The antioxidant activity test yielded an IC₅₀ value of 4,2485 µg/mL, indicating that the extract exhibits very strong antioxidant activity. The high levels of phenolic and flavonoid compounds are believed to play an important role in the radical scavenging activity observed. Based on these findings, it can be concluded that the ethyl acetate extract of andaliman leaves has strong potential to be developed as a natural antioxidant source for applications in the pharmaceutical and health fields.
Downloads
References
Apak, R., Özyürek, M., Güçlü, K., & Çapanoʇlu, E. (2016). Antioxidant activity/capacity measurement. 1. Classification, physicochemical principles, mechanisms, and electron transfer (ET)-based assays. Journal of Agricultural and Food Chemistry, 64(5), 997–1027. https://doi.org/10.1021/acs.jafc.5b04739
Astriani Natalia Br Ginting, Vera Estefania Kaban, Roy Indrianto Bangar, & Daimah W. S. Harahap. (2025). Formulasi dan Uji Aktivitas Antibakteri Gel Minyak Atsiri Rimpang Lengkuas Merah (Alpinia purpurata K. Schum) terhadap Propionibacterium acnes. INSOLOGI: Jurnal Sains Dan Teknologi, 4(1), 75–88. https://doi.org/10.55123/insologi.v4i1.4844
Farmasi, J., Pcjn, N., Irma, P., Lumban, Y., Bangar, R. I., & Kaban, V. E. (2025). Machine Translated by Google Uji Khasiat Ekstrak Etanol Daun Tetanus ( Leea aequata L .) Terhadap Bakteri ( Pseudomonas aeruginosa ) Jurnal Farmasi dan Klinis Nusantara ( PCJN ). Machine Translated by Google. 03(01), 1–7.
Firdaus, S., Rosyidah, M., Permadi, A., Sulistiawati, E., & Wardhana, B. (2024). 19095-46013-1-Pb. Semnasintek, November, 138–143.
Furi, M., Meldayanti, & Octaviani, M. (2024). PENENTUAN KADAR TOTAL FENOLIK DAN FLAVONOID EKSTRAK ETANOL DAN FRAKSI DAUN TERAP (Artocarpus odoratissimus Blanco). Jurnal Penelitian Farmasi Indonesia, 13(1), 57–64. https://doi.org/10.51887/jpfi.v13i1.1765
Hamidu, L., Septiani, V., Meiliana, M. L., & Najib, A. (2025). Potensi Antioksidan Daun Lobi-Lobi (Flacourtia inermis Roxb): Penentuan Kadar Total Flavonoid dan Fenolik. Jurnal Fitofarmaka Indonesia, 12(1), 31. https://doi.org/10.33096/jffi.v12i1.1424
Hasyim Ibroham, M., Jamilatun, S., Dyah Kumalasari, I., Dahlan, A., Ringroad Selatan, J., Banguntapan, K., Bantul, K., & Istimewa Yogyakarta, D. (2022). Seminar Nasional Penelitian LPPM UMJ Website: http://jurnal.umj.ac.id/index.php/semnaslit A REVIEW: POTENSI TUMBUHAN-TUMBUHAN DI INDONESIA SEBAGAI ANTIOKSIDAN ALAMI. Seminar Nasional Penelitian LPPM UMJ, 1(1), 1–13. http://jurnal.umj.ac.id/index.php/semnaslit
Indrianto Bangar, R., Nastira Ningsih, K., Emran Kartasasmita, R., & Insanu, M. (2024). Isolation of α-glucosidase enzyme inhibitor from titanus (Leea aequata L.). Current Research on Biosciences and Biotechnology, 6(1), 2024–2065. https://doi.org/10.5614/crbb.2024.6.1/XCGSRS5W
Kepok, P. (2025). Al-Tamimi Kesmas. 14, 123–133.
M. R. M. Senet, I G. M. A. P. Raharja, I K.T. Darma, K. T. Prastakarini, N. M. A. Dewi, dan I. M. O. A. P. (1907). PENENTUAN KANDUNGAN TOTAL FLAVONOID DAN TOTAL FENOL DARI AKAR KERSEN (Mutingia calabura) SERTA AKTIVITASNYA SEBAGAI ANTIOKSIDAN. 13–18.
Nanda Pratama, A., & Busman, H. (2020). Potensi Antioksidan Kedelai Terhadap Penangkapan Radikal Bebas Potential of Soybean Antioxidant (Glycine Max L) on Capturing Free Radicals. Jurnal Ilmiah Kesehatan Sandi Husada, 11(1), 497–504. https://doi.org/10.35816/jiskh.v10i2.333
Nur, S., Sami, F. J., Awaluddin, A., & Afsari, M. I. A. (2019). Korelasi Antara Kadar Total Flavonoid dan Fenolik dari Ekstrak dan Fraksi Daun Jati Putih (Gmelina Arborea Roxb.) Terhadap Aktivitas Antioksidan. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 5(1), 33–42. https://doi.org/10.22487/j24428744.2019.v5.i1.12034
Simarmata, Y., Thaib, C. M., Nurbaya, S., & Rajagukguk, M. U. O. (2017). Penetapan Kadar Flavonoid Total dari Ekstrak Etanol Daun Andaliman (Zanthoxylum acanthopodium DC. Farmanesia, 4(1), 74–78.
Susanto, F. X. H., Choirunniza, A. N., Subagyo, D. L., & Yanuar, M. R. S. (2025). UJI ANTIOKSIDAN EKSTRAK ETANOL DAUN JAWER KOTOK (Coleus atropurpureus L. Benth) SECARA IN VITRO. Jurnal Farmamedika (Pharmamedica Journal), 10(2), 303–311. https://doi.org/10.47219/ath.v10i2.469
Waruwu, L. D. K. Y., Bangar, R. I., Kaban, V. E., & Sembiring, N. B. (2025). Antioxidant Activity Test of The Ethyl Acetate Fraction of Tetanus Leaf (Leea aequata L.) Using The DPPH Method. PCJN: Pharmaceutical and Clinical Journal of Nusantara, 3(01), 21–25. https://nusantarascientificjournal.com/index.php/pcjn/article/view/88
Wijaya, H., Jubaidah, S., & Rukayyah. (2022). Perbandingan Metode Esktraksi Terhadap Rendemen Ekstrak Batang Turi (Sesbania Grandiflora L.). Indonesian Journal of Pharmacy and Natural Product, 5(1), 1–11.
Wilson Kanggaran, Razoki, R., & Roy Indrianto Bangar Siagian. (2025). Uji Aktivitas Antioksidan Ekstrak Etanol Daun Salam (Syzygium Polyanthum (Wight) Walp.) Dengan Metode DPPH. Jurnal Sains Farmasi Dan Kesehatan, 3(1), 48–55. https://doi.org/10.62379/jfkes.v3i1.2927
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Muhtamilatur Rahma, Roy Indrianto Bangar, Muhammad Yunus
This work is licensed under a Creative Commons Attribution 4.0 International License.
Hak cipta pada setiap artikel adalah milik penulis.
Penulis mengakui bahwa INSOLOGI (Jurnal Sains dan Teknologi) sebagai publisher yang mempublikasikan pertama kali dengan lisensi Creative Commons Attribution 4.0 International License.
Penulis dapat memasukan tulisan secara terpisah, mengatur distribusi non-ekskulif dari naskah yang telah terbit di jurnal ini kedalam versi yang lain, seperti: dikirim ke respository institusi penulis, publikasi kedalam buku, dan lain-lain. Dengan mengakui bahwa naskah telah terbit pertama kali pada INSOLOGI (Jurnal Sains dan Teknologi).
