Formulasi Sediaan Serum Dari Ekstrak Etanol Buah Pepino (Solanum Muricatum) Sebagai Antioksidan dengan Menggunakan Metode DPPH

Abstract

The Solanaceae (nightshade) family of fruits includes the pepino. Protein, beta-carotene, alkaloids, carotenoids, tannins, phenolic compounds, and flavonoids are all present. Vitamins B1, B2, B3, and C are among the minerals and vitamins found in pepino fruit; beta-carotene and vitamin C are antioxidants. The purpose of this study was to ascertain whether the ethanol extract of pepino fruit (Solanum muricatum) could be prepared into a physically and chemically stable serum and to use the DPPH method to calculate the extract's IC50 value for antioxidant activity. Using the DPPH method, the pepino fruit ethanol extract's antioxidant activity was evaluated at 2, 4, 6, 8, and 10 ppm concentrations. The extract was added to serum formulations at 0.5%, 1%, and 2% concentrations. The three serum formulations including the pepino fruit ethanol extract had low stability, but they nonetheless satisfied the overall standards for serum preparations, according to stability data evaluated both before and after a cycle test. The Garnier Vitamin C serum had an IC50 of 6.72 µg/mL for antioxidant activity, while the pepino fruit extract serum had an IC50 of 5.5 µg/mL. The Garnier Vitamin C serum and the pepino fruit extract serum both had extremely strong antioxidant activity