Potensi Antibakteri Ekstrak Lempuyang Gajah (Zingiber zerumbet) terhadap Staphylococcus aureus melalui Pendekatan In Vitro dan In Silico
DOI:
https://doi.org/10.55123/insologi.v5i5.9510Keywords:
Lempuyang Gajah, Staphylococcus Aureus, Antibacteri, ZerumboneAbstract
Staphylococcus aureus infections remain a significant public health issue, exacerbated by increasing antibiotic resistance, highlighting the need to explore new antibacterial agents derived from natural sources. Lempuyang gajah is reported to contain several secondary metabolites with potential antibacterial activity, one of which is the compound zerumbone. This study aims to evaluate the antibacterial potential of Zingeber zerumbet extract against S. aureus using in silico and in vitro approaches. The research was conducted using molecular docking analysis and experimental antibacterial activity testing via the disk diffusion method. The in silico analysis was performed by docking zerumbone to the Clumping Factor A protein (PDB: 1N67) using PyRx 0.8. The Zingeber zerumbet extract was obtained via maceration using 70% ethanol as the solvent, and antibacterial testing was performed at extract concentrations of 25%, 50%, and 75%, with distilled water as the negative control and chloramphenicol as the positive control. The results showed an affinity value of -7.6 kcal/mol for zerumbone toward the target protein. In vitro test results showed that the extract produced average inhibition zones of 13.8 mm, 15.3 mm, and 16.7 mm at concentrations of 25%, 50%, and 75%, respectively. The research results indicate that the compound zerumbone is predicted to have the potential to interact with the Clumping Factor A protein, which is associated with S. aureus virulence. In vitro, the Zingeber zerumbet extract exhibits antibacterial activity against S. aureus.
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